Information

Catalog number

AS05 056

Full name

ExoS, Exoenzyme S

Size

100 µl

Price

588.00 €

Go to shop   

Details

Available ordering format

Liquid in PBS pH 8.0, 0.02% sodium azide

Immunogen

amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Raised in

Hen

Clonality

Polyclonal

Clone

Polyclonal

Purification

Total IgY

How to reconstitute

See included datasheet or contact our support service

Storage condition

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

Secondary antibodies

Recommended dilutions for use

1: 5000 (WB)

Molecular weight (expected | аpparent)

48 kDa

Verified reactivity

Pseudomonas aeruginosa

Possible reactivity

Pseudomonas aeruginosa only

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

Contact Gentaur support center at info@gentaur.com

References

Anantharajah et al. (2016). Inhibition of the Injectisome and Flagellar Type III Secretion Systems by INP1855 Impairs Pseudomonas aeruginosa Pathogenicity and Inflammasome Activation. J Infect Dis. 2016 Jul 13. pii: jiw295.

Scientific context

Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Notes

None

Protein number

Q51449 , PA3841

TAIR number

Refer to NCBI