Information
Catalog number
E-CL-R0073-96T
Full name
Rat BACE1 (Beta Site APP Cleaving Enzyme 1) CLIA Kit
Size
96Test
Price
541.00 €
Go to shopDetails
Detection Type
Colormetric
Target Species
Rat
Target Synonym
ASP2, HSPC104, Beta Secretase 1, Memapsin 2, membrane-associated aspartic protease 2, aspartyl protease 2
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BACE1 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BACE1 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BACE1 . You can calculate the concentration of Rat BACE1 in the samples by comparing the RLU value of the samples to the standard curve.
Sensitivity
18.75pg/mL
Detection Range
31.25~2000pg/mL
Target Name
BACE1
UNIProt ID
NA
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Rat BACE1 (Beta Site APP Cleaving Enzyme 1) CLIA Kit
Type
Sandwich
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus