Menu

Mouse Angiotensin I Converting Enzyme (ACE) CLIA Kit, 96 well plate

Information

Catalog number

EKU08771

Full name

Mouse Angiotensin I Converting Enzyme (ACE) CLIA Kit, 96 well plate

Size

10x96T

Price

7943.00 €

Go to shop   

Details

Verified reactivity

Mus musculus (Mouse)

Detection limits

54.9-40,000 pg/mL

Sensitivity limit

21,5 pg/mL

Samples to be analyzed

Serum, plasma, tissue homogenates, cerebrospinal fluid, cell lysates, cell culture supernates and other biological fluids

Assay duration

2 hours, 40min

Verified applications

CLIA

Assay class

Sandwich

ELISA detection

Chemiluminescent

Protein number

P09470

Alternate protein number

Please refer to SwissProt

Gene number

11421

Gene name

Angiotensin I Converting Enzyme

Alternate gene name

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

Estimated production time

7-11 business days

Shipping requirements

Blue ice

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Use for

8 months

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme (ACE). No significant cross-reactivity or interference between Angiotensin I Converting Enzyme (ACE) and analogues was observed.

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Assay principle

The microplate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensin I Converting Enzyme (ACE) level in the sample or standard.;

Protocol

Please see ELISA's datasheet, otherwise contact us

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Research main area

Enzyme & Kinase;Cardiovascular biology;

Warnings

Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Notes

For research use only. Not for diagnostic procedures.

Description

Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus