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Human UBE2C(Ubiquitin Conjugating Enzyme E2C) ELISA Kit

Information

Catalog number

ELK3129

Full name

Human UBE2C(Ubiquitin Conjugating Enzyme E2C) ELISA Kit

Size

96T

Price

608.00 €

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Details

Alternative Names

UBCH10; Ubiquitin carrier protein C; Ubiquitin-protein ligase C

Assay Type

Sandwich

Sensitivity

0.062ng/mL

Standard

10ng/mL

Detection range

0.156-10ng/mL

Assay length

3h

Research Area

Enzyme & Kinase;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2C (UBE2C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2C (UBE2C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2C (UBE2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Conjugating Enzyme E2C (UBE2C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Description

Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.