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OxiSelect™ DNA Double-Strand Break (DSB) Staining test kit

Information

Catalog number

STA-321

Full name

OxiSelect™ DNA Double-Strand Break (DSB) Staining test kit

Size

100 assays

Price

693.50 €

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Details

We send this reagant on

Blue Ice

The temperatureerature for storage is

-20ºC

SDS or safty data sheet

(STA-321) GHS.pdf

Type of reagent

Oxidative Stress/Damage

Related techniques

Oxidative Stress/ Damage, DNA / RNA Damage and Repair, DNA Double-Strand Break Assay

Use of the reagent

Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect™ DNA Double-Strand Break Staining test kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

Cellular use

Detects phosphorylation of histone H2A.X

Protocol information

See immunofluorescence in about 3 hours

Reagent properties

DNA double-strand break inducer included

Species compatible to

Humans

Use of this test kit or product

Genetics lab

Antigen

a specific protein sequence

Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,50

Molecular weight

in kilo Danlto or kDa

This reagent is purified

0.97