Smith Antigen (SM), 25kD, Clone: Y12, Mouse Monoclonal antibody-Human, Swine, Rabbit, Rat, Drosophila; paraffin, IH/WB/EM/Affinity purification
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Alternative name 1
Smith Antigen (SM), 25kD, Clone: Y12, Mab anti-Human, Swine, Rabbit, Rat, Drosophila; paraffin, IH/WB/EM/Affinity purification
Alternative name 2
Smith Antigen (SM), 25kD, Clone: Y12, Mab antibody-Human, Swine, Rabbit, Rat, Drosophila; paraffin, IH/WB/EM/Affinity purification
Alternative name 3
Smith Antigen (SM), 25kD, Clone: Y12, Mab antibody-Homo sapiens, Swine, Rabbit, Rattus norvegicus, Drosophila; paraffin, IH/WB/EM/Affinity purification
Alternative name 4
anti-Smith Antigen SM 25kD Clone: Y12 Drosophila /EM/Affinity purication
Alternative name 5
Smith Antigen SM 25kD Clone: Y12 Drosophila /EM/Affinity purication
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
A high affinity purification column was use to purify Smith Antigen (SM) 25kD, Clone: Y12, Mouse Monoclonal antibody- Swine, Rabbit, paraffin, IH/ /EM/ purification by accurate-monoclonals by chromatographic size exclusion.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. accurate-monoclonals supplies purification kits and ultra pure reagents.
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Antigens are peptides or recombinant or native dependent on the production method.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by accurate-monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. accurate-monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Mus musculus,Oryctolagus cuniculus,Rattus norvegicus